A multi-protease, multi-dissociation, bottom-up-to-top-down proteomic view of the Loxosceles intermedia venom

A multi-protease, multi-dissociation, bottom-up-to-top-down proteomic view of the Loxosceles intermedia venom

Venoms are a rich source for the discovery of molecules with biotechnological applications, but their analysis is challenging even for state-of-the-art proteomics. Here we report on a large-scale proteomic assessment of the venom of Loxosceles intermedia, the so-called brown spider. Venom was extracted from 200 spiders and fractioned into two aliquots relative to a 10 kDa cutoff mass. Each of t...

Bottom-up, top-down

Bottom-Up Beats Top-Down

In PEPA, the calculation of the transitions enabled by a process accounts for a large part of the time for the state space exploration of the underlying Markov chain. Unlike other approaches based on recursion, we present a new technique that is iterative—it traverses the process’ binary tree from the sequential components at the leaves up to the root. Empirical results shows that this algorith...

Event-Related Potentials of Bottom-Up and Top-Down Processing of Emotional Faces

Introduction: Emotional stimulus is processed automatically in a bottom-up way or can be processed voluntarily in a top-down way. Imaging studies have indicated that bottom-up and top-down processing are mediated through different neural systems. However, temporal differentiation of top-down versus bottom-up processing of facial emotional expressions has remained to be clarified. The present st...

Every sensible extended top-down tree transducer is a multi bottom-up tree transducer

A tree transformation is sensible if the size of each output tree is uniformly bounded by a linear function in the size of the corresponding input tree. Every sensible tree transformation computed by an arbitrary weighted extended top-down tree transducer can also be computed by a weighted multi bottom-up tree transducer. This further motivates weighted multi bottom-up tree transducers as suita...